Human ANTXR1 / TEM8

Mouse

Human (tested or 100% immunogen sequence identity)

Gibbon, Monkey, Cat (at least 90% immunogen sequence identity)

IgG1 Monoclonal

200C1339(SB20)

Unconjugated

Protein G purified

Azide-free

This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.

aa481-497

Human ANTXR1 / TEM8. TEM8 is observed at ~80 kD in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kD and 85 kD double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kD band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail. Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.

• IHC
• IHC - Paraffin (2 - 5 µg/ml)
• ICC (1:10 - 1:500)
• Immunofluorescence (1:10 - 1:500)
• Western blot (1 - 3 µg/ml)

Immunohistochemistry-Paraffin: see Hotchkiss et al 2005 for details. Immunocytochemistry/Immunofluorescence: See Matsuda (2010) for details. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS. No stabilizing proteins added.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9H6X2 NCBI: NM_018153 NP_060623.2