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Anti-ZNRF1 Antibody (aa93-106) LS-C186637

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml Unavailable

Most Popular ZNRF1 Antibodies

Anti-ZNRF1 Antibody (N-Terminus) LS-C110427
Rabbit Polyclonal to Human ZNRF1
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Hamster, Pig, Rabbit, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-ZNRF1 Antibody (clone 1H4) LS-C134112
Mouse Monoclonal [clone 1H4] (IgG1,k) to Human ZNRF1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ZNRF1 Antibody (aa59-86) LS-C160966
Rabbit Polyclonal to Human ZNRF1
Human
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Mouse ZNRF1
Mouse, Bovine, Dog, Guinea pig, Hamster
IHC - Paraffin, ELISA
Unconjugated

Details

Mouse ZNRF1
Goat
Mouse, Bovine, Dog, Guinea pig, Hamster (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin
  • ELISA (1:32000)

Specificity and Use

ZNRF1 antibody was raised against synthetic peptide C-SDSTYAHGNGYQET from an internal region of mouse ZNRF1 (NP_573469.1; NP_001162093.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Cat, Bovine, Guinea pig (100%); Rabbit, Zebra finch (93%).
LS-E27926 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186637. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa93-106
Mouse ZNRF1. This antibody is expected to recognize reported isoforms a and b (NP_573469.1; NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 28kD band observed in Mouse embryo and adult Brain lysates (right panel), consistent with the observed band in transfected N2a cells transiently expressing Znrf1(left panel) Calculated MW of 23.8kD according to NP_573469.1. Recommended concentration: 1-3 ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan IHC: In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10 ug/ml. Data obtained from Dr. Shuji Wakatsuki, National Institute of Neuroscience, Tokyo, Japan.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About ZNRF1

Q8ND25 NM_032268 NP_115644.1

ZNRF1 Antibody, NIN283 Antibody, Zinc/RING finger protein 1 Antibody, Zinc and ring finger 1 Antibody, Nerve injury gene 283 Antibody, Zinc and ring finger protein 1 Antibody

E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL.

Immunohistochemistry

Immunohistochemistry
ZNRF1 antibody (10 ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, Streptavidin-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1:100).

Western blot

Western blot
ZNRF1 antibody N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with (1 ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 3/27/2017

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