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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ZNF224 Antibody (aa127‑155) LS‑C157425
ZNF224 antibody LS-C157425 is an unconjugated rabbit polyclonal antibody to human ZNF224. Validated for WB.
Catalog
Size
Price
LS-C157425-400
400 µl
Unavailable

Popular ZNF224 Products

Detection limit for recombinant GST tagged ZNF224 is 0.1 ng/ml as a capture antibody.
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Immunohistochemistry Dilution at 1:500 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, ELISA

Product Description

ZNF224 antibody LS-C157425 is an unconjugated rabbit polyclonal antibody to human ZNF224. Validated for WB.
About ZNF224
May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells. Q9NZL3 NM_013398 Q9NZL3

ZNF224 Antibody, Bone marrow zinc finger 2 Antibody, KOX22 Antibody, Zinc finger protein 224 Antibody, ZNF255 Antibody, Zinc finger protein 255 Antibody, Zinc finger protein KOX22 Antibody, ZNF27 Antibody, BMZF-2 Antibody, BMZF2 Antibody, Zinc finger protein 233 Antibody, Zinc finger protein 27 Antibody, Zinc finger protein ZNF255 Antibody, Zinc finger 2, bone marrow Antibody


Specifications

Target
Human ZNF224
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E5233 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C157425. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa127-155
Specificity
This ZNF224 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-155 amino acids from the N-terminal region of human ZNF224.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ZNF224 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ZNF224 antibody detected the ZNF224 protein (arrow).
ZNF224 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ZNF224 antibody detected the ZNF224 protein (arrow).

Western blot

ZNF224 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ZNF224 antibody detected the ZNF224 protein (arrow).
ZNF224 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ZNF224 antibody detected the ZNF224 protein (arrow).

Western blot

ZNF224 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ZNF224 antibody detected the ZNF224 protein (arrow).
ZNF224 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ZNF224 antibody detected the ZNF224 protein (arrow).

Western blot

ZNF224 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ZNF224 antibody detected the ZNF224 protein (arrow).
ZNF224 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ZNF224 antibody detected the ZNF224 protein (arrow).


Requested From: 
Date Requested: 6/22/2018

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