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Anti-ZNF207 Antibody (aa88-116) LS-C160836

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular ZNF207 Antibodies

Anti-ZNF207 Antibody (aa223-271) LS-C31142
Rabbit Polyclonal (IgG) to Human ZNF207
Human, Monkey, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Western blot
Unconjugated
Western blot Image
Anti-ZNF207 Antibody (aa360-409) LS-C101451
Rabbit Polyclonal (IgG) to Human ZNF207
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-ZNF207 Antibody (clone 6D7) LS-C133524
Mouse Monoclonal [clone 6D7] (IgG2a,k) to Human ZNF207
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ZNF207
Human
Immunofluorescence, Western blot
Unconjugated

Details

Human ZNF207
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

LS-E3236 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C160836. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa88-116
This ZNF207 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-116 amino acids from the N-terminal region of human ZNF207.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ZNF207

O43670 NM_003457 NP_003448.1

ZNF207 Antibody, D38 Antibody, Zinc finger protein 207 Antibody

Kinetochore-binding protein involved in spindle-assembly checkpoint signaling by regulating mitotic chromosome alignment and mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of A549 cell stained with ZNF207 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZNF207 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZNF207 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
ZNF207 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The ZNF207 antibody detected the ZNF207 protein (arrow).

Requested From: 
Date Requested: 3/29/2017

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