Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Immunofluorescence of monoclonal antibody to ZNF174 on HeLa cell. [antibody concentration 10 ug/ml]
ZNF174 monoclonal antibody (M01), clone 2D7-E9 Western Blot analysis of ZNF174 expression in SW-13.
Western Blot analysis of ZNF174 expression in transfected 293T cell line by ZNF174 monoclonal antibody (M01), clone 2D7-E9.Lane 1: ZNF174 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged ZNF174 is approximately 0.3 ng/ml as a capture antibody.
Western blot analysis of ZNF174 over-expressed 293 cell line, cotransfected with ZNF174 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF174 monoclonal antibody (M01), clone 2D7-E9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.