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ZNF143 / STAF Antibody (aa409‑621) LS‑C185493
STAF antibody LS-C185493 is an unconjugated rabbit polyclonal antibody to human STAF (ZNF143). Validated for ICC, IP and WB.
Catalog
Size
Price
LS-C185493-100
100 µl (0.83 mg/ml)
Unavailable

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Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

STAF antibody LS-C185493 is an unconjugated rabbit polyclonal antibody to human STAF (ZNF143). Validated for ICC, IP and WB.
About ZNF143 / STAF
Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8. P52747 NM_003442 NP_003433.3

ZNF143 Antibody, PHZ-1 Antibody, SBF Antibody, Transcriptional activator Staf Antibody, STAF Antibody, Zinc finger protein 143 Antibody, HStaf Antibody, SPH-binding factor Antibody


Specifications

Target
Human ZNF143 / STAF
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation (1:100 - 1:500)
Immunogen
ZNF143 / STAF antibody was raised against recombinant fragment corresponding to a region within amino acids 409 and 621 of ZNF143 (SwissProt P52747).
Epitope
aa409-621
Specificity
Human STAF / ZNF143
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.

Immunoprecipitation

ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.

Immunoprecipitation

ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.

Immunoprecipitation

ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed A431 using ZNF143 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. ZNF143 antibody diluted at 1:1000.

Immunoprecipitation

ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.
ZNF143 antibody immunoprecipitates ZNF143 protein in IP experiments. IP Sample:293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of ZNF143 protein by 2 ug of ZNF143 antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated ZNF143 protein was detected by ZNF143 antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.


Requested From: 
Date Requested: 6/18/2018

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