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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ZDHHC14 Antibody (aa108‑157) LS‑C101982 Best Seller
ZDHHC14 antibody LS-C101982 is an unconjugated rabbit polyclonal antibody to ZDHHC14 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C101982-50
50 µg
Unavailable

Popular ZDHHC14 Products

Western blot of lysates from mouse brain and mouse cerebellum tissue lysate (from left to right) with ZDHHC14 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.
Species: Human, Mouse
Applications: Western blot
Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human
Applications: ELISA
Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

ZDHHC14 antibody LS-C101982 is an unconjugated rabbit polyclonal antibody to ZDHHC14 from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human ZDHHC14
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila (tested or 100% immunogen sequence identity)
Predicted
Bat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ZDHHC14 antibody was raised against synthetic peptide located between aa108-157 of human ZDHHC14 (Q8IZN3, NP_078906). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Lizard, Xenopus, Stickleback, Zebrafish, Drosophila (100%); Bat, Opossum (92%).
Blocking Peptide
LS-E15697 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101982. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa108-157
Specificity
Human ZDHHC14
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ZDHHC14 antibody LS-C101982 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZDHHC14 antibody LS-C101982 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ZDHHC14 antibody LS-C101982 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZDHHC14 antibody LS-C101982 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ZDHHC14 antibody LS-C101982 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZDHHC14 antibody LS-C101982 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ZDHHC14 antibody LS-C101982 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZDHHC14 antibody LS-C101982 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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