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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ZBTB7B / HcKrox Antibody (Internal) IHC‑plus™ LS‑B9492
Note: This antibody replaces LS-C59279
HcKrox antibody LS-B9492 is an unconjugated rabbit polyclonal antibody to HcKrox (ZBTB7B) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9492-100
100 µl
Unavailable

Popular ZBTB7B / HcKrox Products

Anti-ZBTB7B antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4136 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Detection of Human ZBTB7B by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ZBTB7B antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ZBTB7B was also immunoprecipitated by rabbit anti-ZBTB7B antibody BL13325, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Species: Human
Applications: Western blot, Immunoprecipitation
Immunohistochemical staining of human tissues using anti-Th-POK (human), pAb at 5 ug/ml concentration. . 1. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human uterus (A) and small intestine (B) (40x, brown color). 2. Staining with other rabbit serum (A2, B2) as negative control.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

HcKrox antibody LS-B9492 is an unconjugated rabbit polyclonal antibody to HcKrox (ZBTB7B) from human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ZBTB7B / HcKrox
Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. O15156 NM_015872 NP_056956.2

ZBTB7B Antibody, C-KROX Antibody, HcKROX Antibody, THPOK Antibody, ZFP-67 Antibody, Zinc finger protein 67 homolog Antibody, Zinc finger protein Th-POK Antibody, ZNF857B Antibody, Zinc finger protein 857B Antibody, ZFP67 Antibody, ZBTB15 Antibody


Specifications

Target
Mouse ZBTB7B / HcKrox
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:2500 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ZBTB7B / HcKrox antibody was raised against synthetic peptide corresponding to an internal sequence of mouse cKrox protein.
Epitope
Internal
Specificity
mouse cKrox. A BLAST analysis was used to suggest cross-reactivity with cKrox from mouse, human and rat based on a 100% homology with the immunizing sequence. Reactivity against homologs from other sources is not known
Usage
This antibody has been tested for use in ELISA, Immunohistochemistry with FFPE human tissues and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kD in size corresponding to cKrox by western blotting in the appropriate cell lysate or extract. Lysates from mouse thymocytes are recommended for use as positive controls.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Reconstitution
100 µl deionized water
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-ZBTB7B / HcKrox antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-ZBTB7B / HcKrox antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-ZBTB7B / HcKrox antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-ZBTB7B / HcKrox antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-ZBTB7B / HcKrox antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-ZBTB7B / HcKrox antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-ZBTB7B / HcKrox antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-ZBTB7B / HcKrox antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.


Requested From: 
Date Requested: 6/24/2018

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