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ZBTB48 / HKR3 Antibody (aa468‑517) LS‑C112143
HKR3 antibody LS-C112143 is an unconjugated rabbit polyclonal antibody to HKR3 (ZBTB48) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C112143-50
50 µg
Unavailable

Popular ZBTB48 / HKR3 Products

Antibody
Species: Human, Mouse
Applications: IHC, Western blot
Immunohistochemical analysis of ZBTB48 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human spleen tissue at dilution 1:100
Species: Human
Applications: IHC, ELISA
Immunohistochemistry of paraffin-embedded human spleen tissue at dilution 1:100
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

HKR3 antibody LS-C112143 is an unconjugated rabbit polyclonal antibody to HKR3 (ZBTB48) from human, mouse, rat and other species. Validated for WB.
About ZBTB48 / HKR3
Binds to and regulates the J and/or S elements in MHC II promoter. P10074 NM_005341 NP_005332.1

ZBTB48 Antibody, GLI-Kruppel family member HKR3 Antibody, HKR3 Antibody, Pp9964 Antibody, Protein HKR3 Antibody, Zinc finger protein 855 Antibody, ZNF855 Antibody


Specifications

Target
Human ZBTB48 / HKR3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ZBTB48 / HKR3 antibody was raised against synthetic peptide located between aa468-517 of human ZBTB48 (P10074, NP_005332). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%); Xenopus, Stickleback, Zebrafish (92%).
Blocking Peptide
LS-E21224 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112143. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa468-517
Specificity
Human ZBTB48
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

ZBTB48 antibody LS-C112143 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZBTB48 antibody LS-C112143 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ZBTB48 antibody LS-C112143 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZBTB48 antibody LS-C112143 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ZBTB48 antibody LS-C112143 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZBTB48 antibody LS-C112143 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ZBTB48 antibody LS-C112143 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ZBTB48 antibody LS-C112143 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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