Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
ZBTB33 / Kaiso antibody was raised against fusion protein encompassing the N-terminus of mouse Kaiso.
Reacts with the ~95kD Kaiso protein. Reactivity is confirmed with mouse mammary adenocarcinoma cell line CSML0. Reactivity has been confirmed in MDCK (dog), NBTII (rat), HCT116 (human) and chicken embryo fibroblasts.
Suitable for use in Immunoprecipitation, Immunofluorescence, Western Blot and ELISA. Immunoprecipitation: 10 ug/IP reaction. Immunofluorescence: 10 ug/ml. Western Blot: 1-3 ug/ml. ELISA: 0.1-1 ug/ml. Note: Immunofluorescent assays were performed on cell lines fixed with 100% methanol at -20 ?C for 7 minutes.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
ZBTB33 / Kaiso is a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters.