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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ZBTB33 / Kaiso Antibody (N‑Terminus) LS‑C16123
Kaiso antibody LS-C16123 is an unconjugated mouse monoclonal antibody to Kaiso (ZBTB33) from human, mouse, rat and other species. Validated for ELISA, IF, IP and WB.
Antibody
Catalog
Size
Price
LS-C16123-100
100 µg
$605

Popular ZBTB33 / Kaiso Products

Anti-ZBTB33 / Kaiso antibody IHC of human small intestine, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1116 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Mouse, Human, Rat, Dog, Hamster, Chicken
Applications: IHC, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse, Rat, Dog, Chicken
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human, Mouse, Rat, Dog, Chicken
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

Kaiso antibody LS-C16123 is an unconjugated mouse monoclonal antibody to Kaiso (ZBTB33) from human, mouse, rat and other species. Validated for ELISA, IF, IP and WB.

Specifications

Target
Mouse ZBTB33 / Kaiso
Synonyms
ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348
Host
Mouse
Reactivity
Mouse, Human, Rat, Dog, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
ZBTB33 / Kaiso antibody was raised against fusion protein encompassing the N-terminus of mouse Kaiso.
Epitope
N-Terminus
Specificity
Reacts with the ~95kD Kaiso protein. Reactivity is confirmed with mouse mammary adenocarcinoma cell line CSML0. Reactivity has been confirmed in MDCK (dog), NBTII (rat), HCT116 (human) and chicken embryo fibroblasts.
Usage
Suitable for use in Immunoprecipitation, Immunofluorescence, Western Blot and ELISA. Immunoprecipitation: 10 ug/IP reaction. Immunofluorescence: 10 ug/ml. Western Blot: 1-3 ug/ml. ELISA: 0.1-1 ug/ml. Note: Immunofluorescent assays were performed on cell lines fixed with 100% methanol at -20 ?C for 7 minutes.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About ZBTB33 / Kaiso
ZBTB33 / Kaiso is a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Q86T24 NM_006777 NP_006768.1


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Requested From: United States
Date Requested: 10/21/2018

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