Target
Human ZAP70
Synonyms
ZAP70 | 70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Fusion Protein containing the 2 tandem SH2 domains of human ZAP-70.
Specificity
Recognizes human p70 ZAP. Species cross-reactivity: Does not recognize mouse.
Applications
- Western blot
- Immunoprecipitation
- Flow Cytometry
Usage
Suitable for use in Western Blot, Flow Cytometry and Immunoprecipitation. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitated p70 ZAP from 500 ug of RIPA lysates from Jurkat or Hut-78 cell lines.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee