Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein A purified
IHC - Paraffin (1:500 - 1:2000)
IHC - Frozen (1:500 - 1:2000)
Western blot (1:500 - 1:2000)
Specificity and Use
ZAP70 antibody was raised against ni-NTA purified truncated recombinant ZAP70 expressed in E. coli strain BL21 (DE3).
Recognizes human RBM25.
Suitable for use in ELISA, Western Blot and Immunohistochemistry (Fr) (paraffin-embedded sections). ELISA 1:10000,. Western Blot 1:500-1:2000,. Immunohistochemistry-Paraffin 1:500-1:2000. Immunohistochemistry-Frozen 1:500-1:2000.
Supplied as a liquid
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane.