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Anti-ZAP70 Antibody (C-Terminus, clone ZAP-03) IHC-plus™ LS-B7298

Note: This antibody replaces LS-C44942
Catalog Size Price
LS-B7298-50 50 µg (1 mg/ml) Unavailable

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178 ZAP70 Antibodies

Most Popular ZAP70 Antibodies

Anti-ZAP70 Antibody (clone 3D2A4) LS-C82199
Mouse Monoclonal [clone 3D2A4] (IgG1) to Human ZAP70
Human
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
IHC of paraffin-embedded human lymph showing cytoplasmic localization using ZAP70 antibody with DAB staining.
Anti-ZAP70 Antibody (clone 2F3.2) LS-C87914
Mouse Monoclonal [clone 2F3.2] (IgG2a) to Human ZAP70
Human
IHC, IHC - Paraffin, Western blot
Unconjugated
Formalin-Fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note staining of T-Cells.
Anti-ZAP70 Antibody (phospho-Tyr493) LS-C117511
Rabbit Polyclonal (IgG) to Human ZAP70
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
IHC of paraffin-embedded human breast carcinoma, using ZAP-70 (Phospho-Tyr493) Antibody. The picture on the right is treated with the synthesized peptide.
Anti-ZAP70 Antibody IHC-plus™ LS-B7841
Mouse Monoclonal (IgG1,k) to Human ZAP70
Human
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-ZAP70 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7841 dilution 1:200.
Anti-ZAP70 Antibody (aa337-600, Biotin) LS-C300535
Rabbit Polyclonal (IgG) to Human ZAP70
Human
Western blot
Biotin Conjugated
Western blot of recombinant ZAP70.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Mouse Monoclonal [clone ZAP-03] (IgG1) to Human ZAP70
Human
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human ZAP70
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [ZAP-03]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • Flow Cytometry

Specificity and Use

ZAP70 antibody was raised against bacterially expressed fusion protein corresponding to the C-terminal region of human Zap-70.
C-Terminus
Recognizes human ZAP-70, a 70kD tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukemia.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Membrane permeabilization is required for this application. Western Blot: detects a band of approximately 70kD.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About ZAP70

P43403 NM_207519 P43403

ZAP70 Antibody, 70 kDa zeta-associated protein Antibody, SRK Antibody, STCD Antibody, STD Antibody, Syk-related tyrosine kinase Antibody, Tyrosine-protein kinase ZAP-70 Antibody, TZK Antibody, ZAP-70 Antibody, Zeta-associated protein 70 Antibody

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane.

Immunohistochemistry

Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.
Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.

Requested From: 
Date Requested: 4/23/2018

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