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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ZAP70 Antibody (C‑Terminus, clone ZAP‑03) IHC‑plus™ LS‑B7298
Note: This antibody replaces LS-C44942
ZAP70 antibody LS-B7298 is an unconjugated mouse monoclonal antibody to human ZAP70. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7298-50
50 µg (1 mg/ml)
Unavailable

Popular ZAP70 Products

IHC of paraffin-embedded human lymph showing cytoplasmic localization using ZAP70 antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Formalin-Fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note staining of T-Cells.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded human breast carcinoma, using ZAP-70 (Phospho-Tyr493) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-ZAP70 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7841 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Western blot of recombinant ZAP70.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot

Product Description

ZAP70 antibody LS-B7298 is an unconjugated mouse monoclonal antibody to human ZAP70. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ZAP70
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. P43403 NM_207519 P43403

ZAP70 Antibody, 70 kDa zeta-associated protein Antibody, SRK Antibody, STCD Antibody, STD Antibody, Syk-related tyrosine kinase Antibody, Tyrosine-protein kinase ZAP-70 Antibody, TZK Antibody, ZAP-70 Antibody, Zeta-associated protein 70 Antibody


Specifications

Target
Human ZAP70
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [ZAP-03]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ZAP70 antibody was raised against bacterially expressed fusion protein corresponding to the C-terminal region of human Zap-70.
Epitope
C-Terminus
Specificity
Recognizes human ZAP-70, a 70kD tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukemia.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. Membrane permeabilization is required for this application. Western Blot: detects a band of approximately 70kD.
Presentation
PBS, pH 7.4, 0.09% sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.
Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.

Immunohistochemistry

Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.
Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.

Immunohistochemistry

Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.
Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.

Immunohistochemistry

Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.
Anti-ZAP70 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7298 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/22/2018

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