Target
Human ZAP70
Synonyms
ZAP70 | 70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2.
Specificity
Human ZAP70. This antibody is expected to recognize isoform 1 (NP_001070.2) only.
Applications
- Western blot (0.3 - 1 µg/ml)
- Immunoprecipitation
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 70kD band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kD according to NP_001070.2). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee