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Anti-YWHAH / 14-3-3 Eta Antibody (N-Terminus) IHC-plus™ LS-B3279

Note: This antibody replaces LS-C42372

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular YWHAH / 14-3-3 Eta Antibodies

Anti-YWHAH / 14-3-3 Eta Antibody (N-Terminus) IHC-plus™ LS-B3279
Rabbit Polyclonal (IgG) to Sheep YWHAH / 14-3-3 Eta
Sheep, Human, Mouse, Rat, Bovine, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-YWHAH / 14-3-3 Eta Antibody (aa217-246) LS-C160701
Rabbit Polyclonal to Human YWHAH / 14-3-3 Eta
Human, Mouse
IHC, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-YWHAH / 14-3-3 Eta Antibody (clone 3G12) LS-C180206
Mouse Monoclonal [clone 3G12] (IgG1) to Human YWHAH / 14-3-3 Eta
Human, Mouse, Rat
ICC, Western blot
Unconjugated
Immunocytochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Sheep YWHAH / 14-3-3 Eta
Sheep, Human, Mouse, Rat, Bovine, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated

Details

Sheep YWHAH / 14-3-3 Eta
Rabbit
Sheep, Human, Mouse, Rat, Bovine, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Mammal (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:250)
  • Western blot

Specificity and Use

YWHAH / 14-3-3 Eta antibody was raised against synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 eta.
N-Terminus
Recognizes 14-3-3 eta in all mammals. It is a member of the 14-3-3 family which consists of 30 kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for it's different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 eta is expressed principally in the brain although it is expressed at low levels in other tissues. The protein binds alpha-synuclein, forming part of Lewy bodies in Parkinson's disease and has been linked to early-onset schizophrenia. The eta isoform binds both gremlin 1, which is overexpressed in human cancers, and the DAL-1/Protein 4.1B tumor suppressor. Furthermore the glucocorticoid receptor is positively regulated by 14-3-3- eta. May not react with recombinant proteins that are not N-acetylated.
Immunohistochemistry: LS-B3279 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3279 was determined to be 1:250. Histology Positive Control Tissue: Normal brain tissue Western Blot: LS-B3279 detects a band of approximately 30kD in HEK293 cell lysates.

Packaging

Serum, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About YWHAH / 14-3-3 Eta

Q04917 NM_003405 NP_003396.1

YWHAH Antibody, 14-3-3 eta Antibody, 14-3-3 protein eta Antibody, Protein AS1 Antibody, YWHA1 Antibody

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Immunohistochemistry

Immunohistochemistry
Anti-YWHAH / 14-3-3 Eta antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3279 dilution 1:250.

Immunohistochemistry

Immunohistochemistry
Anti-YWHAH / 14-3-3 Eta antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3279 dilution 1:250.

Requested From: United States
Date Requested: 12/4/2016

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