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YWHAE / 14-3-3 Epsilon

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon

YWHAE / 14-3-3 Epsilon belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene.

Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Synonyms: YWHAE, 14-3-3E, 14-3-3 epsilon, 14-3-3 protein epsilon, MDCR, MDS, KCIP-1
Target Sequences: NM_006761 NP_006752.1 P62258

☰ Filters
Antibodies (38)
Proteins (11)
ELISA Kits (2)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (2)
Primary (38)
Recombinant (8)
Sandwich (2)
YWHAE / 14-3-3 Epsilon (51)
Human (38)
Mouse (25)
Rat (23)
Bat (9)
Bovine (10)
Chicken (10)
Chimpanzee (9)
Dog (9)
Guinea pig (9)
Horse (9)
Monkey (10)
Rabbit (9)
Sheep (10)
Zebrafish (10)
IHC (12)
IHC-P (7)
WB (37)
Flo (2)
ELISA (13)
ICC (4)
IF (8)
IP (3)
Peptide-ELISA (2)
rabbit (35)
mouse (3)
Product Group
IHCPlus (1)
IgG (17)
IgG1 (1)
IgG2a,k (1)
IgG2b,k (1)
monoclonal mc (3)
polyclonal pc (35)
07 (1)
5A5 (1)
AT4F8 (1)
96-Well Microplate (1)
96-Well Strip Plate (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
Preservative Free (2)
Unconjugated (26)
C-Terminus (13)
180-260 aa, C-terminal (1)
Asp238 (1)
aa1-255 (1)
aa206-255 (1)
No (51)
Cell Lysates (2)
Plasma (1)
Serum (1)
Tissue Homogenates (2)
6His, N-terminus (2)
GST (2)
GST, N-terminus (2)
Myc-DDK (Flag) (3)
Human (9)
Mouse (1)
293T Cells (1)
E. coli (5)
HEK 293 Cells (4)
Wheat Germ Extract (1)
Purified (5)
Low endotoxin level (2)
YWHAE / 14-3-3 Epsilon Antibody - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: Mouse Brain Tissue lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 14-3-3 Zeta Recombinant Protein Lane 2.: 14-3-3 Beta Recombinant Protein Lane 3.: 14-3-3 Epsilon Recombinant Protein Lane 4.: 14-3-3 Eta Recombinant Protein Lane 5.: 14-3-3 Gamma Recombinant Protein Lane 6.: 14-3-3 Sigma Recombinant Protein Lane 7.: 14-3-3 Tau Recombinant Protein
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YWHAE / 14-3-3 Epsilon Mouse anti-Human Monoclonal (AT4F8) Antibody
Human
ELISA, Flo, ICC, WB
Unconjugated
50 µl/$303; 100 µl/$365
YWHAE / 14-3-3 Epsilon Protein
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E. coli
None
100 µg/$411; 500 µg/$985; 20 µg/$276
YWHAE / 14-3-3 Epsilon Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$460
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Human
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100 µg/$331
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$294; 100 µg/$360
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA, IHC, IHC-P, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA, IHC, IHC-P, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA, IHC, IHC-P, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
YWHAE / 14-3-3 Epsilon Antibody - Western blot of 14-3-3 epsilon antibody
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (180-260 aa, C-terminal) Antibody
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
YWHAE / 14-3-3 Epsilon Antibody - Immunofluorescence staining of YWHAE in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human YWHAE polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, IP, WB
Unconjugated
100 µl/$412
YWHAE / 14-3-3 Epsilon Antibody - Anti-YWHAE rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: SHSY5Y Whole Cell Lysate. Lane D: SW480 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 32 kDa. (We are unsure as to the identity of these extra bands.)
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Human
ELISA, ICC, IF, IP, WB
Unconjugated, Preservative Free
100 µl/$412
YWHAE / 14-3-3 Epsilon Antibody - IHC of 14-3-3 (D238) pAb in paraffin-embedded human brain tissue.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (Asp238) Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
50 µl/$321; 100 µl/$403
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E. coli
GST, N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
56kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,157
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded Human colon cancer using YWHAE Polyclonal Antibody at dilution of 1:100.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded Human colon cancer using YWHAE Polyclonal Antibody at dilution of 1:60.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
YWHAE / 14-3-3 Epsilon Antibody - Immunofluorescent analysis of 14-3-3 epsilon staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Chicken, Zebrafish, Mouse, Rat, Sheep, Bovine, Human, Monkey
ICC, IF, WB
Unconjugated
100 µl/$299
YWHAE / 14-3-3 Epsilon Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemical analysis of 14-3-3 epsilon staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Mouse Polyclonal Antibody
Mouse, Rat
IHC, IHC-P, WB
Unconjugated
100 µl/$321
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YWHAE / 14-3-3 Epsilon Mouse anti-Human Monoclonal (aa1-255) (5A5) Antibody
Human
ELISA, WB
Unconjugated
100 µl/$586
YWHAE / 14-3-3 Epsilon Antibody - Western blot of YWHAE pAb in extracts from A375 cells.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
YWHAE / 14-3-3 Epsilon Antibody - Western blot analysis of extracts from HepG2 cells and Jurkat cells, using 14-3-3 e antibody.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
YWHAE / 14-3-3 Epsilon Antibody - Western blot analysis of lysates from HepG2 and Jurkat cells, using 14-3-3 epsilon Antibody. The lane on the right is blocked with the synthesized peptide.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal (aa206-255) Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
Select
YWHAE / 14-3-3 Epsilon Rabbit anti-Mouse Polyclonal Antibody
Mouse, Rat
WB
Unconjugated
100 µg/$357
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YWHAE / 14-3-3 Epsilon Mouse anti-Human Monoclonal (Preservative Free) (07) Antibody
Human
ELISA
Unconjugated, Preservative Free
100 µl/$412
YWHAE / 14-3-3 Epsilon Antibody - YWHAE / 14-3-3 Epsilon antibody Western blot of Mouse Kidney lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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YWHAE / 14-3-3 Epsilon Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$424
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).