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Antibodies > YWHAE / 14-3-3 Epsilon > LS-C370574
 
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Catalog Number Size Price
LS-C370574-50 50 µg $294 
LS-C370574-100 100 µg $360 
YWHAE / 14-3-3 Epsilon Antibody
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded human lung tissue using antibody at 1: 100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
YWHAE / 14-3-3 Epsilon Antibody - IHC image of YWHAE Antibody diluted at 1:1000 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
YWHAE / 14-3-3 Epsilon Antibody - IHC image of YWHAE Antibody diluted at 1:1000 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
YWHAE / 14-3-3 Epsilon Antibody - Immunofluorescence staining of HepG2 cells with YWHAE Antibody at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
YWHAE / 14-3-3 Epsilon Antibody - Western Blot Positive WB detected in: A375 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, Mouse brain tissue All lanes: YWHAE antibody at 3.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 27 kDa Observed band size: 30 kDa
YWHAE / 14-3-3 Epsilon Antibody
YWHAE / 14-3-3 Epsilon Antibody - Western blot. All lanes: YWHAE antibody at 2 ug/ml. Lane 1: HeLa whole cell lysate Lane 2: mouse brain tissue. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 29 kDa. Observed band size: 29 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
YWHAE / 14-3-3 Epsilon Antibody
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded human lung tissue using antibody at 1: 100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
YWHAE / 14-3-3 Epsilon Antibody - Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
YWHAE / 14-3-3 Epsilon Antibody - IHC image of YWHAE Antibody diluted at 1:1000 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
YWHAE / 14-3-3 Epsilon Antibody - IHC image of YWHAE Antibody diluted at 1:1000 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
YWHAE / 14-3-3 Epsilon Antibody - Immunofluorescence staining of HepG2 cells with YWHAE Antibody at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
YWHAE / 14-3-3 Epsilon Antibody - Western Blot Positive WB detected in: A375 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, Mouse brain tissue All lanes: YWHAE antibody at 3.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 27 kDa Observed band size: 30 kDa
YWHAE / 14-3-3 Epsilon Antibody
YWHAE / 14-3-3 Epsilon Antibody - Western blot. All lanes: YWHAE antibody at 2 ug/ml. Lane 1: HeLa whole cell lysate Lane 2: mouse brain tissue. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 29 kDa. Observed band size: 29 kDa. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Rabbit anti‑Human YWHAE / 14‑3‑3 Epsilon Antibody (IHC, WB) LS‑C370574

Polyclonal Rabbit anti‑Human YWHAE / 14‑3‑3 Epsilon Antibody (IHC, WB) LS‑C370574

Submit Review LSBio Guarantee
Antibody:
YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
FITC HRP Biotin
Price
Catalog Number
$294
LS-C370574-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
YWHAE / 14-3-3 Epsilon Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
FITC HRP Biotin

Specifications

Description
14-3-3 Epsilon antibody LS-C370574 is an unconjugated rabbit polyclonal antibody to 14-3-3 Epsilon (YWHAE) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
Target
Human YWHAE / 14-3-3 Epsilon
Synonyms
YWHAE | 14-3-3E | 14-3-3 epsilon | 14-3-3 protein epsilon | MDCR | MDS | KCIP-1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human 14-3-3 protein epsilon protein.
Specificity
Human YWHAE / 14-3-3 Epsilon
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About YWHAE / 14-3-3 Epsilon
P62258 NM_006761 NP_006752.1

Publications (0)

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Featured Products

YWHAE / 14-3-3 Epsilon Antibody - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: Mouse Brain Tissue lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 14-3-3 Zeta Recombinant Protein Lane 2.: 14-3-3 Beta Recombinant Protein Lane 3.: 14-3-3 Epsilon Recombinant Protein Lane 4.: 14-3-3 Eta Recombinant Protein Lane 5.: 14-3-3 Gamma Recombinant Protein Lane 6.: 14-3-3 Sigma Recombinant Protein Lane 7.: 14-3-3 Tau Recombinant Protein
YWHAE / 14‑3‑3 Epsilon Antibody (clone AT4F8) LS‑C755593
Species: Human
Applications: ICC, Western blot, Flow Cytometry, ELISA
YWHAE / 14-3-3 Epsilon Protein
Human YWHAE / 14-3-3 Epsilon Protein (Recombinant) - LS-G3598
Source: E. coli
Tag: None
Product
Human YWHAE / 14-3-3 Epsilon Protein (Recombinant GST, N-terminus) (Met1-Gln255) - LS-G39538
Source: E. coli
Tag: GST, N-terminus
Product
IGFBPL1 Antibody LS‑C36896
Species: Human
Applications: Western blot, ELISA
Tetracycline ELISA Kit
Tetracycline (Competitive EIA) ELISA Kit - LS-F74107
Reactivity: All species
Range: 123.5-10000 pg/ml
ISG15 ELISA Kit
Mouse ISG15 (Sandwich ELISA) ELISA Kit - LS-F17499
Reactivity: Mouse
Range: 78-5000 pg/ml
See all YWHAE / 14-3-3 Epsilon Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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