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Anti-YWHAB / 14-3-3 Beta Antibody (aa1-246, clone J2E9) IHC-plus™ LS-B2958

Note: This antibody replaces LS-C93822

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular YWHAB / 14-3-3 Beta Antibodies

Anti-YWHAB / 14-3-3 Beta Antibody (aa1-246, clone J2E9) IHC-plus™ LS-B2958
Mouse Monoclonal [clone J2E9] (IgG2a,k) to Human YWHAB / 14-3-3 Beta
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-YWHAB / 14-3-3 Beta Antibody IHC-plus™ LS-B4792
Rabbit Polyclonal to Human YWHAB / 14-3-3 Beta
Human, Zebrafish
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone J2E9] (IgG2a,k) to Human YWHAB / 14-3-3 Beta
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human YWHAB / 14-3-3 Beta
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [J2E9]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

YWHAB / 14-3-3 Beta antibody was raised against recombinant human 14-3-3 beta (1-246aa) purified from E. coli.
aa1-246
Immunohistochemistry: LS-B2958 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2958 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About YWHAB / 14-3-3 Beta

P31946 NM_003404 NP_003395.1

YWHAB Antibody, 14-3-3 alpha Antibody, 14-3-3 Beta Antibody, 14-3-3 protein beta/alpha Antibody, GW128 Antibody, Protein 1054 Antibody, HS1 Antibody, KCIP-1 Antibody, YWHAA Antibody

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.

Immunohistochemistry

Immunohistochemistry
Anti-14-3-3 Beta antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2958 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-14-3-3 Beta antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2958 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-14-3-3 Beta antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2958 concentration 5 ug/ml.

Western blot

Western blot
Cell lysates of HeLa(30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 beta (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: United States
Date Requested: 12/8/2016

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