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Anti-Yeast SUMO Antibody LS-C59540

Ordering

Wt. Vol. Conc. Price
500 µg 0.5 ml 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Western blot Image
Anti-Yeast SUMO Antibody LS-C186427
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Anti-Yeast SUMO Antibody (aa1-97) LS-C204271
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100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Yeast SUMO
Yeast
Western blot, ELISA
Unconjugated

Details

Yeast SUMO
Rabbit
Yeast (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA (1:1000 - 1:5000)

Specificity and Use

Yeast SUMO antibody was raised against recombinant yeast SUMO protein.
anti-Rabbit Serum
This purified polyclonal antibody reacts yeast SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A 12 kD band corresponding to yeast SUMO is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
0.5 ml Phosphate Buffered Saline (PBS)
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

Publications (1)

1
Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis. Vizeacoumar FJ, van Dyk N, S Vizeacoumar F, Cheung V, Li J, Sydorskyy Y, Case N, Li Z, Datti A, Nislow C, Raught B, Zhang Z, Frey B, Bloom K, Boone C, Andrews BJ. The Journal of cell biology. 2010 188:69-81. [PubMed:20065090] [PMC:PMC2812844]

About Yeast SUMO


Western blot

Western blot
Anti-SUMO Antibody - Western Blot. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins. Each sample was run twice. The left lanes each contain 2 ug E. coli expressed and purified SUMO-GFP fusion proteins after incubation with lysed cells (50 ug total protein) for 1 h. The right lanes contain the same fusion proteins incubated with the lysate in the presence of 2% SDS. After probing with anti-GFP antibodies the membranes were stripped of antibody using SDS-DTT solution for 30 m at 60° C and were then re-probed using the anti-SUMO antibody at a 1:1000 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG (code # LS-C60943) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/4/2016

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