Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Yeast (tested or 100% immunogen sequence identity)
Western blot (1:5000)
Specificity and Use
Yeast Rad9 antibody was raised against synthetic peptide corresponding to phosphorylated form of aa 1125-1139 of 1309 of yeast Rad9 protein conjugated to KLH.
the phosphorylated form of yeast Rad9 at the pS1260 residue
This phospho specific polyclonal antibody was tested by ELISA. Data from ELISA indicates the antibody is reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. No reactivity is expected against the human or mouse analogs of RAD9. Reactivity against RAD9 from other sources is unknown. Although not tested, this antibody is likely functional by WB, IHC and IP. This product has been assayed against 0.1 ug of phosphorylated peptide (pS1129) in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:5000 is suggested for this product. Minimal reactivity was detected against the nonphosphorylated form of the immunizing peptide. This antibody appears to be specific for the active form (phosphorylated) of the protein. Researchers should determine optimal titers for other applications.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.