(tested or 100% immunogen sequence identity)
- Western blot (1:100 - 1:500)
- ELISA (1:5000)
Yeast Rad9 antibody was raised against whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa1125-1139 of 1309 of yeast Rad9 protein (KLH coupled).
Recognizes both the phosphorylated and non-phosphorylated forms of RAD9. No reactivity is expected against human and mouse homologs.
Suitable for use in Western Blot and ELISA. Western Blot: 1:100-1:500. ELISA: 1:5000. 0.1 ug of immunizing peptide was used in a standard capture ELISA using TMB as a substrate for 30 minutes at RT.
PBS, pH 7.2, 0.01% Sodium Azide, No stabilizing proteins added
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.