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Yeast Rad9 Antibody (aa1125‑1139) LS‑C17554 Best Seller
Antibody
Yeast Rad9 antibody LS-C17554 is an unconjugated rabbit polyclonal antibody to yeast Yeast Rad9. Validated for ELISA and WB.
Catalog
Size
Price
LS-C17554-100
100 µg
Unavailable

Popular Yeast Rad9 Products

Antibody
Species: Yeast
Applications: Western blot, ELISA
Antibody
Species: Yeast
Applications: Western blot, ELISA
Anti-Rad9 Antibody- Western Blot. Affinity purified phospho-specific antibody to yeast Rad9 at pS1260 was used at a 1:200 dilution incubated overnight at 4° C to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa ~60 kD) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 was by treatment with ATP and Rad53 kinase. Rad53 kinase autophosphorylates and appears cross reactive as it is detected on the blot as 90 and 110 kD bands (asterisk). Detection occurred using a 1:5000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Species: Yeast
Applications: Western blot, ELISA
Antibody
Species: Yeast
Applications: Western blot, ELISA

Product Description

Yeast Rad9 antibody LS-C17554 is an unconjugated rabbit polyclonal antibody to yeast Yeast Rad9. Validated for ELISA and WB.

Specifications

Target
Yeast Rad9
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:100 - 1:500)
  • ELISA (1:5000)
Immunogen
Yeast Rad9 antibody was raised against whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa1125-1139 of 1309 of yeast Rad9 protein (KLH coupled).
Epitope
aa1125-1139
Specificity
Recognizes both the phosphorylated and non-phosphorylated forms of RAD9. No reactivity is expected against human and mouse homologs.
Usage
Suitable for use in Western Blot and ELISA. Western Blot: 1:100-1:500. ELISA: 1:5000. 0.1 ug of immunizing peptide was used in a standard capture ELISA using TMB as a substrate for 30 minutes at RT.
Presentation
PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/19/2018

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