Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
YBX1 Antibody, CBF-A Antibody, DBPB Antibody, CSDB Antibody, DNA-binding protein B Antibody, MDR-NF1 Antibody, NSEP-1 Antibody, NSEP1 Antibody, Y box binding protein 1 Antibody, Y-box transcription factor Antibody, Y-box-binding protein 1 Antibody, YB-1 Antibody, BP-8 Antibody, CSDA2 Antibody, EFI-A Antibody, Enhancer factor I subunit A Antibody, YB1 Antibody
Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors By similarity. Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'.
Fluorescent confocal image of U251 cell stained with YBX1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). YBX1 immunoreactivity is localized to Cytoplasm significantly.
Western blot of YBX1(arrow) using rabbit polyclonal YBX1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies).
YBX1 Antibody western blot of 293 cell line lysates (35 ug/lane). The YBX1 antibody detected the YBX1 protein (arrow).
Requested From: United States
Date Requested: 2/24/2017