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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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YAP / YAP1 Antibody (phospho‑Ser127) LS‑C199738
YAP1 antibody LS-C199738 is an unconjugated rabbit polyclonal antibody to YAP1 (YAP) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199738-50
50 µl
Unavailable
LS-C199738-100
100 µl
Unavailable

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Product Description

YAP1 antibody LS-C199738 is an unconjugated rabbit polyclonal antibody to YAP1 (YAP) from human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
About YAP / YAP1
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. P46937 NM_006106 NP_006097.2

YAP1 Antibody, 65 kDa Yes-associated protein Antibody, YAP Antibody, Yes-associated protein beta Antibody, Yes-associated protein delta Antibody, Yorkie homolog Antibody, YAP2 Antibody, Yes-associated protein 1 Antibody, Yes-associated protein 2 Antibody, YKI Antibody, YAP65 Antibody


Specifications

Target
Human YAP / YAP1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
YAP / YAP1 antibody was raised against synthetic peptide derived from human YAP around the phosphorylation site of Ser127.
Epitope
pSer127
Specificity
YAP (Phospho-Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine127.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/25/2018

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