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Anti-YAP / YAP1 Antibody (aa351-400) IHC-plus™ LS-B3561

Note: This antibody replaces LS-C81566

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular YAP / YAP1 Antibodies

Anti-YAP / YAP1 Antibody (clone 2F12) IHC-plus™ LS-B4494
Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human YAP / YAP1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-YAP / YAP1 Antibody (clone 2H1) IHC-plus™ LS-B4495
Mouse Monoclonal [clone 2H1] (IgG2a,k) to Human YAP / YAP1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human YAP / YAP1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human YAP / YAP1
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

YAP / YAP1 antibody was raised against synthetic peptide located between aa351-400 of human YAP1 (P46937, NP_001123617). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish (100%).
LS-E18240 - Lyophilized - 100 µg - $145.00
aa351-400
Human YAP1
Immunohistochemistry: LS-B3561 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3561 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About YAP / YAP1

P46937 NM_006106 NP_006097.2

YAP1 Antibody, 65 kDa Yes-associated protein Antibody, YAP Antibody, Yes-associated protein beta Antibody, Yes-associated protein delta Antibody, Yorkie homolog Antibody, YAP2 Antibody, Yes-associated protein 1 Antibody, Yes-associated protein 2 Antibody, YKI Antibody, YAP65 Antibody

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-YAP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3561 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-YAP1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3561 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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