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Anti-XRCC5 / Ku80 Antibody (C-Terminus) LS-C353017

Catalog Size Price
LS-C353017-100 100 µl (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human XRCC5 / Ku80
Human, Monkey
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human XRCC5 / Ku80
Rabbit
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)

Specificity and Use

XRCC5 / Ku80 antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Ku80.
C-Terminus
Recognizes endogenous levels of Ku80 protein.

Packaging

PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About XRCC5 / Ku80

P13010 NM_021141 NP_066964.1

XRCC5 Antibody, 86 kDa subunit of Ku antigen Antibody, CTC85 Antibody, CTCBF Antibody, DNA repair protein XRCC5 Antibody, G22P2 Antibody, KARP-1 Antibody, Ku autoantigen, 80kDa Antibody, NFIV Antibody, KARP1 Antibody, Ku86 Antibody, KUB2 Antibody, TLAA Antibody, Nuclear factor IV Antibody, KU80 Antibody, Thyroid-lupus autoantigen Antibody

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.

Immunohistochemistry

Immunohistochemical analysis of Ku80 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of Ku80 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Immunofluorescence

Immunofluorescent analysis of Ku80 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of Ku80 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of Ku80 expression in HeLa (A); A673 (B); A549 (C); COS7 (D) whole cell lysates.
Western blot analysis of Ku80 expression in HeLa (A); A673 (B); A549 (C); COS7 (D) whole cell lysates.

Requested From: 
Date Requested: 4/22/2018

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