Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IHC - Frozen (1:200 - 1:1000)
ICC (1:200 - 1:1000)
Western blot (1:200 - 1:1000)
Specificity and Use
XRCC5 / Ku80 antibody was raised against synthetic peptide aa557-570, HEDGPTAKKLKTEQ, corresponding to Human Ku80 (BSA). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset (93%).
Recognizes Ku80 at 82.6kD. Species cross-reactivity: Human and rodent.
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 (ECL). Immunohistochemistry: Frozen sections. Not tested for paraffin. 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.
PBS, pH 7.2, 40% glycerol. No preservative added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
XRCC5 Antibody, 86 kDa subunit of Ku antigen Antibody, CTC85 Antibody, CTCBF Antibody, DNA repair protein XRCC5 Antibody, G22P2 Antibody, KARP-1 Antibody, Ku autoantigen, 80kDa Antibody, NFIV Antibody, KARP1 Antibody, Ku86 Antibody, KUB2 Antibody, TLAA Antibody, Nuclear factor IV Antibody, KU80 Antibody, Thyroid-lupus autoantigen Antibody
Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.