Target
Human XPNPEP2
Synonyms
XPNPEP2 | APP2 | MAmP | Membrane-bound APP | X-prolyl aminopeptidase 2 | Xaa-Pro aminopeptidase 2 | X-Pro aminopeptidase 2 | Membrane-bound AmP
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived recombinant human APP2, aa 23-650.
Specificity
Recognizes human X-Prolyl Aminopeptidase P2. Cross-reactivity: recombinant mouse APP2 ~25% and no cross-reactivity with recombinant human XPNPEP1.
Applications
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation (25 µg/ml)
- ELISA
Usage
Suitable for use ELISA, Immunoprecipitation and Western Blot. ELISA (Direct): 0.5-1.0 ug/ml. Detection limit for rhAPP2 is approximately 2 ng/well. Western Blot: 1-2 ug/ml. Detection limit for rhAPP2 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 ug/ml. Immunoprecipitate rhAPP2 from conditioned cell culture medium. The recovered rhAPP2 can be detected in Western blots using a polyclonal antibody.
Presentation
Lyophilized from Sterile Solution PBS, 5% Trehalose
Reconstitution
Reconstitute with sterile 40-50% glycerol, PBS.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee