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Anti-XCR1 Antibody (Extracellular Domain) IHC-plus™ LS-A158

Catalog Size Price
LS-A158-50 50 µg (1 mg/ml) Unavailable
Same day shipping In Stock - Same day shipping

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100% Guaranteed In Stock
Rabbit Polyclonal to Human XCR1
Human
IHC - Paraffin
Unconjugated

Details

Human XCR1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

IHC - Paraffin (2.5 µg/ml)

Specificity and Use

XCR1 antibody was raised against synthetic 19 amino acid peptide from 3rd extracellular domain of human XCR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (89%).
LS-E28336 - Liquid - 50 µg 1 mg/ml - $145.00
Immunizing peptide used to generate LS-A158. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Extracellular Domain
Human XCR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A158 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A158 was determined to be 2.5 ug/ml.

Packaging

PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
For research use only.

About XCR1

P46094 NM_005283 NP_005274.1

XCR1 Antibody, CCXCR1 Antibody, Chemokine (C motif) receptor 1 Antibody, Chemokine XC receptor 1 Antibody, G protein-coupled receptor 5 Antibody, GPR5 Antibody, Lptn receptor Antibody, Lymphotactin receptor Antibody, XC chemokine receptor 1 Antibody, G-protein coupled receptor 5 Antibody

XCR1 is a chemokine receptor belonging to the G protein-coupled receptor superfamily. The family members are characterized by the presence of 7 transmembrane domains and numerous conserved amino acids. This receptor is most closely related to RBS11 and the MIP1-alpha/RANTES receptor. It transduces a signal by increasing the intracellular calcium ions level. The viral macrophage inflammatory protein-II is an antagonist of this receptor and blocks signaling.

Immunohistochemistry

Immunohistochemistry
Anti-XCR1 antibody LS-A158 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: 
Date Requested: 11/20/2017

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