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Anti-WT5 / POU6F2 Antibody (aa22-34) LS-C113014


Wt. Vol. Conc. Price
100 µg - - Unavailable

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Goat Polyclonal to Human WT5 / POU6F2
Human, Monkey, Mouse, Rat, Bovine, Rabbit, Chicken
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Human WT5 / POU6F2
Human, Monkey, Mouse, Rat, Bovine, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:16000)

Specificity and Use

WT5 / POU6F2 antibody was raised against synthetic peptide RSEMNAELRGEDK-C from the N-terminus of human POU6F2 (NP_009183.3; NP_001159490.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Bovine, Rabbit, Chicken (100%); Opossum (92%).
LS-E27365 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C113014. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human POU6F2. This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 65kDa band observed in Mouse Eye lysates (calculated MW of 73.3kDa according to Human NP_009183.3 and Mouse NP_778171.2). Recommended concentration: 0.3-1 ug/ml.


Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About WT5 / POU6F2

P78424 NM_007252 NP_009183.3

POU6F2 Antibody, POU class 6 homeobox 2 Antibody, WTSL Antibody, Wilms tumor suppressor locus Antibody, RPF-1 Antibody, WT5 Antibody

WT5 / POU6F2 is a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways.

Western blot

Western blot
POU6F2 antibody (0.3 ug/ml) staining of Mouse Eye lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 3/27/2017

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