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Anti-WT1 / Wilms Tumor 1 Antibody (N-Terminus) LS-C101671

Catalog	Size	Price
LS-C101671-50	50 µg	Unavailable

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Anti-WT1 / Wilms Tumor 1 Antibody (aa346-375, PE) LS-C224666

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Format:	PE Conjugated

Antibody:	Rabbit Polyclonal (IgG) to Human WT1 / Wilms Tumor 1
Reactivity:	Human
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

WT1 / Wilms Tumor antibody LS-C101671 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human WT1 / Wilms Tumor 1
Host:	Rabbit
Reactivity:	Human (tested or 100% immunogen sequence identity)
Predicted:	Monkey (at least 90% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Failed:

IHC - Paraffin

Specificity and Use

Immunogen:

Synthetic peptide from N-Terminus of human WT1 (NP_000369). Percent identity by BLAST analysis: Human (100%); Gorilla, Marmoset (92%).

Blocking Peptide:

LS-E13623 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101671. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

N-Terminus

Specificity:

Human WT1

Usage:

Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About WT1 / Wilms Tumor 1

Uniprot: P19544 NCBI: NM_024426 NP_077744.3

Synonyms: WT1 Antibody, GUD Antibody, EWS-WT1 Antibody, Amino-terminal domain of EWS Antibody, WAGR Antibody, WIT-2 Antibody, Wilms tumor protein Antibody, AWT1 Antibody, Wilms tumor 1 Antibody, WT33 Antibody, NPHS4 Antibody

Description: WT1 / Wilms Tumor 1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues.

(More About WT1 / Wilms Tumor 1)

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Western blot

WT1 / Wilms Tumor antibody LS-C101671 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/24/2018

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