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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WT1 / Wilms Tumor 1 Antibody (aa16‑30) LS‑C55689
Wilms Tumor 1 antibody LS-C55689 is an unconjugated goat polyclonal antibody to human Wilms Tumor 1 (WT1). Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C55689-100
100 µg
Unavailable

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Product Description

Wilms Tumor 1 antibody LS-C55689 is an unconjugated goat polyclonal antibody to human Wilms Tumor 1 (WT1). Validated for Peptide-ELISA and WB.
About WT1 / Wilms Tumor 1
WT1 / Wilms Tumor 1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. P19544 NM_024426 NP_077744.3

WT1 Antibody, GUD Antibody, EWS-WT1 Antibody, Amino-terminal domain of EWS Antibody, WAGR Antibody, WIT-2 Antibody, Wilms tumor protein Antibody, AWT1 Antibody, Wilms tumor 1 Antibody, WT33 Antibody, NPHS4 Antibody


Specifications

Target
Human WT1 / Wilms Tumor 1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:4000) & (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Failed Applications
  • IHC - Paraffin
Immunogen
Synthetic peptide QDPASTCVPEPASQH from the N-terminus of human WT1 (NP_000369.3; NP_077742.2; NP_077743.2; NP_077744.3). Percent identity with other species by BLAST analysis: Human (100%), Mouse (93%), Rat (93%), Marmoset (87%), Monkey (80%).
Blocking Peptide
LS-E26134 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55689. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa16-30
Specificity
Human WT1. This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Antibody (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/20/2018

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