Human WNT8B / Wnt 8b

Rat

Human, Mouse (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

E. coli-derived rmWnt-8b.

Selected for its ability to detect rmWnt-8b in direct ELISAs and Western Blots. In these formats, this antibody shows no cross-reactivity with rhWnt-2, -7a, -7b, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, or -9b.

• Western blot
• ELISA

Western Blot: This antibody can be used at 1-2 ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit for rmWnt-8b is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. This antibody was used at 5 ug/ml to detect Wnt-8b in cell extracts. Western Blot buffers are listed below. Wash Buffer Blocking Buffer Blotting Buffer 25 mM Tris, pH 7.4 5% nonfat dry milk 2% nonfat dry milk. 0.5 M NaCl in Wash Buffer in Wash Buffer 0.05% Tween 20. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. The detection limit for rmWnt-8b is approximately 5 ng/well.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q93098 NCBI: NM_003393 NP_003384.2