WNT8A antibody LS-A8519 is an unconjugated rabbit polyclonal antibody to WNT8A from human, bat, horse and other species. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Human, Monkey, Bat, Horse
(tested or 100% immunogen sequence identity)
Bovine, Dog (at least 90% immunogen sequence identity)
Also available conjugated with AP
- IHC - Paraffin (10 - 20 µg/ml)
- (applications tested for the base form of this product only)
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WNT8A antibody was raised against synthetic 14 amino acid peptide from internal region of human WNT8A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Elephant, Panda, Horse (100%); Bovine, Dog (93%); Rabbit (86%).
- Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A8519. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human WNT8A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A8519 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A8519 was determined to be 10-20 ug/ml.
PBS, 0.1% Sodium Azide
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
For research use only.