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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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WNT3A Antibody (Leu256) LS‑C76876 Best Seller
Antibody
WNT3A antibody LS-C76876 is an unconjugated rabbit monoclonal antibody to WNT3A from human and mouse. Validated for IP and WB.
Catalog
Size
Price
LS-C76876-100
100 µl
Unavailable

Popular WNT3A Products

The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Anti-WNT3A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5876 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Guinea pig, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-WNT3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5896 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bovine, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

Product Description

WNT3A antibody LS-C76876 is an unconjugated rabbit monoclonal antibody to WNT3A from human and mouse. Validated for IP and WB.
About WNT3A
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. P56704 NM_033131 NP_149122.1

WNT3A Antibody, Protein Wnt-3a Antibody


Specifications

Target
Human WNT3A
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation (1:50)
Immunogen
WNT3A antibody was raised against synthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a (KLH).
Epitope
Leu256
Specificity
Recognizes transfected Wnt3a protein in ul cells and the conditioned medium from ul/Wnt3a cells. Species sequence homology: human, mouse and rat.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.
Presentation
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol, 0.02% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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