(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- Immunoprecipitation (1:50)
WNT3A antibody was raised against synthetic peptide corresponding to residues surrounding Leu256 of human Wnt3a (KLH).
Recognizes transfected Wnt3a protein in ul cells and the conditioned medium from ul/Wnt3a cells. Species sequence homology: human, mouse and rat.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol, 0.02% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.