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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WNT3A Antibody (aa19‑352, clone 3A6) LS‑C93819
WNT3A antibody LS-C93819 is an unconjugated mouse monoclonal antibody to human WNT3A. Validated for ELISA and WB.
Catalog
Size
Price
LS-C93819-100
100 µg (1 mg/ml)
Unavailable

Popular WNT3A Products

Antibody
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: Western blot, ELISA
Anti-WNT3A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5876 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Guinea pig, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-WNT3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5896 concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bovine, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

Product Description

WNT3A antibody LS-C93819 is an unconjugated mouse monoclonal antibody to human WNT3A. Validated for ELISA and WB.
About WNT3A
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. P56704 NM_033131 NP_149122.1

WNT3A Antibody, Protein Wnt-3a Antibody


Specifications

Target
Human WNT3A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [3A6]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:2000)
  • ELISA
Failed Applications
  • IHC - Paraffin
Immunogen
WNT3A antibody was raised against recombinant human Wnt3a (19-352aa) purified from E. coli. NCBI Accession No.: NP_149122.
Epitope
aa19-352
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000-1:2000.
Presentation
PBS, pH 7.4, 0.1% sodium azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western blot

The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western blot

The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Western blot

The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The lysates of A549 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.


Requested From: 
Date Requested: 6/18/2018

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