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IHCPlus™ WNT3 Antibody aa315‑329 Polyclonal IHC,Peptide‑ELISA,WB LS‑B3241

Catalog Number Size Price
LS-B3241-50 50 µg (0.5 mg/ml) $425 
Anti-WNT3 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-WNT3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-WNT3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
WNT3 antibody (1µg/ml) staining of lysate of cell line HEK293 (35µg protein in RIPA buffer). Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-WNT3 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-WNT3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-WNT3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
WNT3 antibody (1µg/ml) staining of lysate of cell line HEK293 (35µg protein in RIPA buffer). Detected by chemiluminescence.
Antibody (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ WNT3 Antibody aa315‑329 Polyclonal IHC,Peptide‑ELISA,WB LS‑B3241

Note: This antibody replaces LS-C55693
Antibody:
Goat Polyclonal to Human WNT3
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3241-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human WNT3
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
WNT3 antibody LS-B3241 is an unconjugated goat polyclonal antibody to WNT3 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human WNT3
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
Blocking Peptide
LS-E26098 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3241. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa315-329
Specificity
Human WNT3.
Usage
Immunohistochemistry: LS-B3241 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3241 was determined to be 3.75 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WNT3
P56703 NM_030753 NP_110380.1

Validation

Anti-WNT3 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
IHC Validated
Anti-WNT3 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-WNT3 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

Anti-WNT3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
IHC Validated
Anti-WNT3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-WNT3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

Anti-WNT3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
IHC Validated
Anti-WNT3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.
Anti-WNT3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.

Publications (1)

Thalamic WNT3 Secretion Spatiotemporally Regulates the Neocortical Ribosome Signature and mRNA Translation to Specify Neocortical Cell Subtypes. Kraushar ML, Viljetic B, Wijeratne HR, Thompson K, Jiao X, Pike JW, Medvedeva V, Groszer M, Kiledjian M, Hart RP, Rasin MR. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2015 35:10911-26. (WB; Human)

Reviews (0)

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Requested From: United States
Date Requested: 9/22/2019