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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WNT3 Antibody (clone 1F6) LS‑C175674
WNT3 antibody LS-C175674 is an unconjugated mouse monoclonal antibody to human WNT3. Validated for IHC and WB.
Catalog
Size
Price
LS-C175674-100
100 µl (0.92 mg/ml)
Unavailable

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Product Description

WNT3 antibody LS-C175674 is an unconjugated mouse monoclonal antibody to human WNT3. Validated for IHC and WB.
About WNT3
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. P56703 NM_030753 NP_110380.1

WNT3 Antibody, Proto-oncogene Int-4 homolog Antibody, Proto-oncogene Wnt-3 Antibody, INT4 Antibody, WNT-3 proto-oncogene protein Antibody


Specifications

Target
Human WNT3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1F6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
WNT3 antibody was raised against human recombinant protein fragment corresponding to amino acids 128-355 of human WNT3 (NP_110380) produced in E. coli.
Specificity
Human WNT3
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.


Requested From: 
Date Requested: 6/22/2018

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