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Anti-WEE1 Antibody (phospho-Ser123) LS-C96996

Catalog Size Price
LS-C96996-400 400 µl Unavailable

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Anti-WEE1 Antibody (phospho-Ser123) LS-C96996
Rabbit Polyclonal to Human WEE1
Human
IHC - Paraffin, Dot Blot
Unconjugated
Immunohistochemistry Image
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Anti-WEE1 Antibody (C-Terminus) LS-C329106
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Anti-WEE1 Antibody LS-C415508
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100% Guaranteed Publications (1)
Rabbit Polyclonal to Human WEE1
Human
IHC - Paraffin, Dot Blot
Unconjugated

Details

Human WEE1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Dot Blot (1:500)

Specificity and Use

LS-E7329 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C96996. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
pSer123
This Wee1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S123 of human Wee1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Persistence of the cell-cycle checkpoint kinase Wee1 in SadA- and SadB-deficient neurons disrupts neuronal polarity. Mller M, Lutter D, Pschel AW. Journal of cell science. 2010 123:286-94. [PubMed:20026642]

About WEE1

P30291 NM_003390 NP_003381.1

WEE1 Antibody, Wee1 homolog Antibody, WEE1+ homolog Antibody, WEE1A Antibody, Wee1A kinase Antibody, WEE1hu Antibody, Wee1 tyrosine kinase Antibody, Wee1-like protein kinase Antibody, Wee1+ (S. pombe) homolog Antibody, WEE1 homolog (S. pombe) Antibody

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma

Dot Blot

Dot Blot
Dot blot of anti-Phospho-Wee1-S123 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibodies working concentration was 0.5ug per ml.

Requested From: 
Date Requested: 6/23/2017

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