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Anti-WEE1 Antibody (C-Terminus) LS-C11059

Ordering

Wt. Vol. Conc. Price
100 µg - - Unavailable

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85 WEE1 Antibodies

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Anti-WEE1 Antibody (phospho-Ser123) LS-C96996
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Immunohistochemistry Image
Anti-WEE1 Antibody (aa604-634) LS-C101209
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Anti-WEE1 Antibody (aa144-173) LS-C101210
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Anti-WEE1 Antibody (phospho-Ser53) LS-C199732
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Anti-WEE1 Antibody LS-C415508
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Xenopus WEE1
Xenopus
Western blot, Immunoprecipitation, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Xenopus WEE1
Rabbit
Xenopus (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

WEE1 antibody was raised against a 16 amino acid synthetic peptide derived from the extreme C-terminus of Xenopus Wee1.
C-Terminus
Specific for the ~68kD Wee1 protein (also referred to as Wee1A). Species cross-reactivity: Xenopus.
Suitable for use in ELISA, Western Blot, Immunoprecipitation. ELISA: 0.1-1.0 ug/ml. Western Blot: 1-3 ug/ml. Immunoprecipitation (native): 5-10 ug/IP reaction.

Packaging

PBS, pH 7.2, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About WEE1

P30291 NM_003390 NP_003381.1

WEE1 Antibody, Wee1 homolog Antibody, WEE1+ homolog Antibody, WEE1A Antibody, Wee1A kinase Antibody, WEE1hu Antibody, Wee1 tyrosine kinase Antibody, Wee1-like protein kinase Antibody, Wee1+ (S. pombe) homolog Antibody, WEE1 homolog (S. pombe) Antibody

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur.

Requested From: 
Date Requested: 4/28/2017

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