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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WEE1 Antibody (aa144‑173) LS‑C101210
WEE1 antibody LS-C101210 is an unconjugated rabbit polyclonal antibody to WEE1 from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C101210-400
400 µl
Unavailable

Popular WEE1 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
Species: Human
Applications: IHC, IHC - Paraffin, Dot Blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, ELISA, Dot Blot

Product Description

WEE1 antibody LS-C101210 is an unconjugated rabbit polyclonal antibody to WEE1 from human and mouse. Validated for IHC and WB.
About WEE1
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. P30291 NM_003390 NP_003381.1

WEE1 Antibody, Wee1 homolog Antibody, WEE1+ homolog Antibody, WEE1A Antibody, Wee1A kinase Antibody, WEE1hu Antibody, Wee1 tyrosine kinase Antibody, Wee1-like protein kinase Antibody, Wee1+ (S. pombe) homolog Antibody, WEE1 homolog (S. pombe) Antibody


Specifications

Target
Human WEE1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9501 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101210. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa144-173
Specificity
This WEE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the Central region of human WEE1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

WEE1 Antibody (A159) western blot of A431, Jurkat cell line lysates (35 ug/lane). The WEE1 antibody detected the WEE1 protein (arrow).
WEE1 Antibody (A159) western blot of A431, Jurkat cell line lysates (35 ug/lane). The WEE1 antibody detected the WEE1 protein (arrow).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

WEE1 Antibody (A159) western blot of A431, Jurkat cell line lysates (35 ug/lane). The WEE1 antibody detected the WEE1 protein (arrow).
WEE1 Antibody (A159) western blot of A431, Jurkat cell line lysates (35 ug/lane). The WEE1 antibody detected the WEE1 protein (arrow).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

WEE1 Antibody (A159) western blot of A431, Jurkat cell line lysates (35 ug/lane). The WEE1 antibody detected the WEE1 protein (arrow).
WEE1 Antibody (A159) western blot of A431, Jurkat cell line lysates (35 ug/lane). The WEE1 antibody detected the WEE1 protein (arrow).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

WEE1 Antibody (A159) western blot of A431, Jurkat cell line lysates (35 ug/lane). The WEE1 antibody detected the WEE1 protein (arrow).
WEE1 Antibody (A159) western blot of A431, Jurkat cell line lysates (35 ug/lane). The WEE1 antibody detected the WEE1 protein (arrow).


Requested From: 
Date Requested: 6/19/2018

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