Target
Human WDR9 / BRWD1
Synonyms
BRWD1 | C21orf107 | N143 | Transcriptional unit N143 | WD repeat-containing protein 9 | WD repeat domain 9 | WDR9 | WD repeat protein WDR9-form2
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence RQNCKGDSQPNKSIR, from the internal region of the protein sequence according to NP_061836.2NP_387505.1NP_001007247.1.
Specificity
Human BRWD1 / WDR9. This antibody is expected to recognize reported isoforms A and B (NP_061836.2; NP_387505.1).
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Preliminary experiments gave an approx 100kD band in Human Heart and Skeletal Muscle lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 263kD according to NP_061836.2. The 100kD band was successfully blocked by incubation with the immunizing peptide. We found identical results with an antibody generated to a different part of the protein (EB11491).
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee