LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-WDR77 / MEP50 Antibody (clone 3F10) IHC-plus™ LS-B4555

Note: This antibody replaces LS-C105173

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular WDR77 / MEP50 Antibodies

Anti-WDR77 / MEP50 Antibody (clone 3F10) IHC-plus™ LS-B4555
Mouse Monoclonal [clone 3F10] (IgG1,k) to Human WDR77 / MEP50
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-WDR77 / MEP50 Antibody (aa1-343, APC) LS-C226704
Mouse Monoclonal (IgG1) to Human WDR77 / MEP50
Human
IHC, Western blot, ELISA
APC Conjugated
Anti-WDR77 / MEP50 Antibody (C-Terminus) IHC-plus™ LS-B14116
Rabbit Polyclonal to Human WDR77 / MEP50
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3F10] (IgG1,k) to Human WDR77 / MEP50
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human WDR77 / MEP50
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3F10]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

WDR77 / MEP50 antibody was raised against wDR77 (AAH01679, 1 a.a. ~ 343 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control HeLa. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human tonsil. Sandwich ELISA (Recombinant protein).

Packaging

PBS, pH 7.4. Sourced in Ascites.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About WDR77 / MEP50

Q9BQA1 NM_024102 NP_077007.1

WDR77 Antibody, Androgen receptor cofactor p44 Antibody, HKMT1069 Antibody, MEP-50 Antibody, Methylosome protein 50 Antibody, p44 Antibody, MEP50 Antibody, Nbla10071 Antibody, RP11-552M11.3 Antibody, WD repeat domain 77 Antibody

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation.

Immunohistochemistry

Immunohistochemistry
Anti-WDR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4555 concentration 20 ug/ml.

Western blot

Western blot
WDR77 monoclonal antibody (M01), clone 3F10 Western blot of WDR77 expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged WDR77 is approximately 3 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/5/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number