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WDR77 / MEP50

WD repeat domain 77

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

Gene Name: WD repeat domain 77
Synonyms: WDR77, Androgen receptor cofactor p44, HKMT1069, MEP-50, Methylosome protein 50, p44, MEP50, Nbla10071, RP11-552M11.3, WD repeat domain 77
Target Sequences: NM_024102 NP_077007.1 Q9BQA1

☰ Filters
Antibodies (31)
Proteins (5)
Over-Expression Lysate (2)
Primary (31)
Recombinant (3)
WDR77 / MEP50 (36)
Human (31)
Mouse (9)
Rat (2)
Bovine (3)
Chimpanzee (3)
Dog (3)
Gibbon (3)
Guinea pig (3)
Monkey (3)
Orangutan (3)
IHC (9)
IHC-P (5)
WB (24)
ELISA (10)
ICC (4)
IF (5)
IP (2)
rabbit (17)
mouse (14)
Product Group
IHCPlus (2)
IgG (14)
IgG1 (10)
IgG1,k (1)
monoclonal mc (14)
polyclonal pc (17)
3F10 (1)
OTI1F7 (2)
OTI4A6 (2)
OTI5E9 (2)
OTI5H9 (2)
Azide-free (1)
Biotin Conjugated (2)
Carrier-free (4)
FITC Conjugated (2)
HRP Conjugated (2)
Preservative Free (1)
Unconjugated (25)
aa1-250 (8)
N-Terminus (4)
aa1-312 (4)
aa292-342 (2)
aa1-260 (1)
aa1-343 (1)
aa1-50 (1)
No (36)
GST (1)
His, N-Terminal (1)
His-SUMO (1)
Myc-DDK (Flag) (2)
Human (4)
293T Cells (1)
E. coli (2)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (1)
WDR77 / MEP50 Antibody - Anti-WDR77 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (3F10) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal Antibody
Mouse, Rat, Human
ICC, WB
Unconjugated
50 µl/$356; 100 µl/$461
WDR77 / MEP50 Protein
Select
E. coli
His-SUMO
49.5 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
WDR77 / MEP50 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
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WDR77 / MEP50 Rabbit anti-Human Polyclonal (aa292-342) Antibody
Mouse, Human
IF, IHC, IHC-P
Unconjugated
50 µl/$460
WDR77 / MEP50 Antibody - Immunohistochemistry of paraffin-embedded human pancreas using antibody at 1:100 dilution.
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WDR77 / MEP50 Rabbit anti-Human Polyclonal (aa1-260) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$294; 100 µl/$360
Select
WDR77 / MEP50 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$341
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-343) (Azide-free) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated, Azide-free
100 µg/$705
WDR77 / MEP50 Antibody - Immunofluorescence staining of WDR77 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human WDR77 polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
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WDR77 / MEP50 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Human
ICC, IF, IHC-P, WB
Unconjugated, Preservative Free
100 µl/$412
WDR77 / MEP50 Antibody - Immunohistochemistry of paraffin-embedded human prostate.
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WDR77 / MEP50 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
Select
WDR77 / MEP50 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
WDR77 / MEP50 Antibody - Detection of Human WDR77/MEP50 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-WDR77/MEP50 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
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WDR77 / MEP50 Rabbit anti-Human Polyclonal (aa292-342) Antibody
Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
WDR77 / MEP50 Antibody - Western blot analysis of extracts of various cells.
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WDR77 / MEP50 Mouse anti-Human Monoclonal Antibody
Human
ICC, WB
Unconjugated
50 µl/$341; 100 µl/$431; 200 µl/$539
WDR77 / MEP50 Antibody - Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100, next using WDR77 mouse monoclonal antibody (dilution 1:100).
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WDR77 / MEP50 Mouse anti-Human Monoclonal Antibody
Mouse, Human
ICC, WB
Unconjugated
100 µl/$391
WDR77 / MEP50 Antibody - Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0. 1% Triton X-100, next using WDR77 mouse mAb (dilution 1:100).
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WDR77 / MEP50 Mouse anti-Human Monoclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µg/$332; 100 µg/$404; 200 µg/$505
WDR77 / MEP50 Antibody - Detection of Human WDR77/MEP50 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-WDR77/MEP50 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
Select
WDR77 / MEP50 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Human
IHC, IP
Unconjugated
10 µg/$295; 100 µl/$514
WDR77 / MEP50 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY WDR77. (Right lane) cDNA for 48 hrs and lysed
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-250) (OTI4A6) Antibody
Human
WB
Unconjugated
100 µl/$434
WDR77 / MEP50 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY WDR77. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR77. (1:2000)
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-250) (Carrier-free) (OTI4A6) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
WDR77 / MEP50 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY WDR77. (Right lane) cDNA for 48 hrs and lysed
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-250) (OTI1F7) Antibody
Human
WB
Unconjugated
100 µl/$434
WDR77 / MEP50 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY WDR77. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR77. (1:2000)
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-250) (Carrier-free) (OTI1F7) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
WDR77 / MEP50 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY WDR77. (Right lane) cDNA for 48 hrs and lysed
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-250) (OTI5E9) Antibody
Human
WB
Unconjugated
100 µl/$434
WDR77 / MEP50 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY WDR77. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR77. (1:2000)
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-250) (Carrier-free) (OTI5E9) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
WDR77 / MEP50 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY WDR77. (Right lane) cDNA for 48 hrs and lysed
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-250) (OTI5H9) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$434
WDR77 / MEP50 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY WDR77. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR77. (1:500)
Select
WDR77 / MEP50 Mouse anti-Human Monoclonal (aa1-250) (Carrier-free) (OTI5H9) Antibody
Mouse, Human
WB
Unconjugated, Carrier-free
100 µg/$511
WDR77 / MEP50 Antibody - Western blot analysis of Hela lncap NIH/3T3 K562 and 231 cell  using WDR77 Polyclonal Antibody at dilution of 1:500
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WDR77 / MEP50 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
WDR77 / MEP50 Antibody - Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using WDR77 Antibody at dilution of 1:100
Select
WDR77 / MEP50 Rabbit anti-Human Polyclonal (aa1-312) Antibody
Human
ELISA
Unconjugated
50 µg/$294; 100 µg/$360
Viewing 1-25 of 36 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).