Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG2a) to Bovine WC1 Surface Antigen
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Bovine WC1 Surface Antigen
Bovine, Pig (tested or 100% immunogen sequence identity)
Protein G purified
IHC - Frozen
Flow Cytometry (1:10 - 1:50)
Specificity and Use
Con A stimulated bovine lymphocytes.
Recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform. The bovine WC1 cell surface antigen is expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. Immunoprecipitates a 215 kD molecule from bovine cells, and also recognizes the swine homolog of WC1, which is a 180 kD molecule. In pigs the 180 kD molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8.
Flow Cytometry: Use10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.