Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone H74] (IgG1,k) to Human VTCN1 / B7-H4
IHC - Paraffin, Flow Cytometry
Human VTCN1 / B7-H4
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [H74]
IHC - Paraffin (20 µg/ml)
Specificity and Use
VTCN1 / B7-H4 antibody was raised against human VTCN1
This H74 antibody has been tested by flow cytometric analysis of transient hB7-H4 transfected cells. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Aqueous buffer, 0.09% sodium azide. Sourced from ascites.
VTCN1 Antibody, B7 superfamily member 1 Antibody, B7 Superfamily, member 1 Antibody, B7h.5 Antibody, B7S1 Antibody, B7H4 Antibody, PRO1291 Antibody, VCTN1 Antibody, RP11-229A19.4 Antibody, B7 family member, H4 Antibody, B7-H4 Antibody, B7X Antibody, Protein B7S1 Antibody
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Anti-VTCN1 / B7-H4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4055 concentration 20 ug/ml.
Anti-VTCN1 / B7-H4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4055 concentration 20 ug/ml.
Staining of non-transfected 293T cells with 0.125 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.125 ug of Purified anti-human B7-H4 (H74) (colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Total cells were used for analysis.
Staining of human B7-H4 transfected 293T cells with 0.125 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.125 ug of Purified anti-human B7-H4 (H74) (colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Total cells were used for analysis.