Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
VSV-g Tag antibody was raised against this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G).
This affinity purified antibody is directed against VSV-G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
This affinity purified antibody has been tested for use in ELISA and by immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluor-escence assay we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
Western blot of Rabbit anti-VSV-G antibody. Lane 1: 12 Epitope Tag Protein Marker Lysate - MB-301-0100. Lane 2: none. Load: ~10 ug per lane. Primary antibody: VSV-G antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 3% BSA-TBS 2H at RT. Predicted/Observed size: ~50 kDa for VSV-G. Other band(s): VSV-G splice variants and isoforms.