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Anti-VSV-g Tag Antibody (aa501-511) LS-C154225

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml Unavailable

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Details

VSV-g Tag
Rabbit
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC (1:200 - 1:500)
  • Immunofluorescence (1:200 - 1:500)
  • Western blot (1:200 - 1:2000)
  • ELISA

Specificity and Use

VSV-g Tag antibody was raised against this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G).
aa501-511
This affinity purified antibody is directed against VSV-G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
This affinity purified antibody has been tested for use in ELISA and by immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluor-escence assay we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
For research use only.

About VSV-g Tag


Western blot

Western blot
Western blot of Rabbit anti-VSV-G antibody. Lane 1: 12 Epitope Tag Protein Marker Lysate - MB-301-0100. Lane 2: none. Load: ~10 ug per lane. Primary antibody: VSV-G antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight 649 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 3% BSA-TBS 2H at RT. Predicted/Observed size: ~50 kDa for VSV-G. Other band(s): VSV-G splice variants and isoforms.
 

Requested From: 
Date Requested: 3/30/2017

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