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Anti-VRK1 Antibody (clone 4F9) LS-C198432

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VRK1 Antibodies

Anti-VRK1 Antibody (Internal, APC) LS-C284196
Rabbit Polyclonal (IgG) to Human VRK1
Human, Mouse
Western blot, ELISA
APC Conjugated
Anti-VRK1 Antibody (Internal, FITC) LS-C284199
Rabbit Polyclonal (IgG) to Human VRK1
Human, Mouse
Western blot, ELISA
FITC Conjugated
Anti-VRK1 Antibody (aa46-292, FITC) LS-C304467
Rabbit Polyclonal (IgG) to Human VRK1
Human
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4F9] (IgG2a,k) to Human VRK1
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA, RNA interference
Unconjugated

Details

Human VRK1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [4F9]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA
  • RNA interference

Specificity and Use

VRK1 antibody was raised against vRK1 (NP_003375, aa287-396 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human VRK1
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About VRK1

Q99986 NM_003384 NP_003375.1

VRK1 Antibody, 51PK Antibody, PCH1A Antibody, Vaccinia related kinase 1 Antibody, Vaccinia-related kinase 1 Antibody, PCH1 Antibody

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to VRK1 on HeLa cell . [antibody concentration 10 ug/ml]

Western blot

Western blot
Western Blot analysis of VRK1 expression in transfected 293T cell line by VRK1 monoclonal antibody (M02), clone 4F9.Lane 1: VRK1 transfected lysate(45.5 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of VRK1 transfected lysate using anti-VRK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with VRK1 monoclonal antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged VRK1 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of VRK1 over-expressed 293 cell line, cotransfected with VRK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK1 monoclonal antibody (M02) clone 4F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
 

Requested From: United States
Date Requested: 12/10/2016

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