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Catalog Number Size Price
LS-C98613-400 400 µl $393 
Vimentin Antibody - Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Vimentin Antibody - Fluorescent confocal image of SY5Y cells stained with Vimentin antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Vimentin primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
Vimentin Antibody - Western blot of Vimentin antibody in NCI-H460 cell line lysates (35 ug/lane). Vimentin (arrow) was detected using the purified antibody.
Vimentin Antibody - Vimentin Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Vimentin Antibody - Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Vimentin Antibody - Fluorescent confocal image of SY5Y cells stained with Vimentin antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Vimentin primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
Vimentin Antibody - Western blot of Vimentin antibody in NCI-H460 cell line lysates (35 ug/lane). Vimentin (arrow) was detected using the purified antibody.
Vimentin Antibody - Vimentin Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human Vimentin Antibody (aa430‑457, IHC, IF, WB) LS‑C98613

Polyclonal Rabbit anti‑Human Vimentin Antibody (aa430‑457, IHC, IF, WB) LS‑C98613

Antibody:
Vimentin Rabbit anti-Human Polyclonal (aa430-457) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98613-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Vimentin Rabbit anti-Human Polyclonal (aa430-457) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Vimentin antibody LS-C98613 is an unconjugated rabbit polyclonal antibody to human Vimentin (aa430-457). Validated for Flow, IF, IHC and WB.
Target
Human Vimentin
Synonyms
VIM | Vimentin | vimenin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Hamster, Pig, Chicken, Xenopus, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa430-457
Specificity
This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Vimentin
P08670 NM_003380 NP_003371.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024