Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Vimentin is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification.
Vimentin in Human Skin. Vimentin was detected in immersion fixed paraffin-embedded sections of human skin using 10 ug/ml Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Vimentin in A549 Human Cell Line. Vimentin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/ml TGF-beta for 48 hours using Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).