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Anti-VEGFR-1/2 Antibody (aa800-900) IHC-plus™ LS-B668

Note: This antibody replaces LS-C2931

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VEGFR-1/2 Antibodies

Anti-VEGFR-1/2 Antibody (aa800-900) IHC-plus™ LS-B668
Rabbit Polyclonal to Mouse VEGFR-1/2
Mouse, Human, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Mouse VEGFR-1/2
Mouse, Human, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Mouse VEGFR-1/2
Rabbit
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:250 - 1:1000)

Specificity and Use

VEGFR-1/2 antibody was raised against a synthetic peptide made to an internal region of the murine VEGFR1 protein sequence (between residues 800-900). There is 87% identity between the immunogen used for this production and the VEGFR-2 protein. Percent identity by BLAST analysis: Mouse, Rat, Human (100%).
aa800-900
This targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
Immunohistochemistry: LS-B668 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B668 was determined to be 5 ug/ml.

Packaging

Tris glycine, 150 mM sodium chloride, 0.05% sodium azide.
Store at +4°C. DO NOT FREEZE.
For research use only.

About VEGFR-1/2


Immunohistochemistry

Immunohistochemistry
Anti-VEGFR1/2 antibody IHC of human lung, endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B668 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

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