Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IHC - Paraffin (5 µg/ml)
Western blot (1:250 - 1:1000)
Specificity and Use
VEGFR-1/2 antibody was raised against a synthetic peptide made to an internal region of the murine VEGFR1 protein sequence (between residues 800-900). There is 87% identity between the immunogen used for this production and the VEGFR-2 protein. Percent identity by BLAST analysis: Mouse, Rat, Human (100%).
This targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
Immunohistochemistry: LS-B668 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B668 was determined to be 5 ug/ml.
Tris glycine, 150 mM sodium chloride, 0.05% sodium azide.
Anti-VEGFR1/2 antibody IHC of human lung, endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B668 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.